The involvement of the mitochondrial peroxiredoxin PRXIIF in defining physiological differences between orthodox and recalcitrant seeds of two Acer species.
Identifieur interne : 002458 ( Main/Exploration ); précédent : 002457; suivant : 002459The involvement of the mitochondrial peroxiredoxin PRXIIF in defining physiological differences between orthodox and recalcitrant seeds of two Acer species.
Auteurs : Ewelina Ratajczak [Pologne] ; Elke Str Her [Allemagne] ; Marie-Luise Oelze [Allemagne] ; Ewa M. Kalemba [Pologne] ; Stanis Awa Pukacka [Pologne] ; Karl-Josef Dietz [Allemagne]Source :
- Functional plant biology : FPB [ 1445-4416 ] ; 2013.
Abstract
Norway maple (Acer platanoides L., orthodox) and sycamore (Acer pseudoplatanus L., recalcitrant) belong to the same genus and grow under similar climatic conditions, but their seeds differ in their tolerance to desiccation. The initial water content (WC) of the seeds used in this study was 50%, and they were dried to 40, 20 and 7%. The mitochondrial peroxiredoxin IIF (PRXIIF) was identified in seeds of both species by immunoblotting. Semiquantitative RT-PCR analyses indicated that the transcript level of PRXIIF in both types of seeds increased during different stages of desiccation and was higher in seeds of Norway maple than in sycamore. General proteome analyses showed important differences between orthodox and recalcitrant seeds. In sycamore seeds that had been desiccated to a 7% WC, the number of protein spots and the levels of those spots were lower than in desiccation-tolerant Norway maple seeds. Post-translational modifications of PRXIIF in seeds at a 50% WC were detected via 2D electrophoresis and subsequent western blot analysis. The detected shift in the pI values (± 0.3) in A. pseudoplatanus was possibly caused by phosphorylation because several potential phosphorylation sites were predicted in silico for that protein. The gene and amino acid sequences were obtained and aligned with known sequences of other plant PRXIIF genes and proteins. High values of sequence identity were noted between the PRXIIF protein sequences of Acer species, Populus trichocarpa Torr. & A. Gray and Arabidopsis thaliana (L.) Heynh. The involvement of PRXIIF in defining the physiological differences between desiccation-tolerant and desiccation-sensitive Acer seeds is discussed in the context of its role in mitochondrial redox homeostasis.
DOI: 10.1071/FP13002
PubMed: 32481169
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Kalemba</name><affiliation wicri:level="1"><nlm:affiliation>Institute of Dendrology, Polish Academy of Sciences, Seed Biochemistry Laboratory, Parkowa 5, 62-035 Kórnik, Poland.</nlm:affiliation><country xml:lang="fr">Pologne</country><wicri:regionArea>Institute of Dendrology, Polish Academy of Sciences, Seed Biochemistry Laboratory, Parkowa 5, 62-035 Kórnik</wicri:regionArea><wicri:noRegion>62-035 Kórnik</wicri:noRegion></affiliation></author><author><name sortKey="Pukacka, Stanis Awa" sort="Pukacka, Stanis Awa" uniqKey="Pukacka S" first="Stanis Awa" last="Pukacka">Stanis Awa Pukacka</name><affiliation wicri:level="1"><nlm:affiliation>Institute of Dendrology, Polish Academy of Sciences, Seed Biochemistry Laboratory, Parkowa 5, 62-035 Kórnik, Poland.</nlm:affiliation><country xml:lang="fr">Pologne</country><wicri:regionArea>Institute of Dendrology, Polish Academy of Sciences, Seed Biochemistry Laboratory, Parkowa 5, 62-035 Kórnik</wicri:regionArea><wicri:noRegion>62-035 Kórnik</wicri:noRegion></affiliation></author><author><name sortKey="Dietz, Karl Josef" sort="Dietz, Karl Josef" uniqKey="Dietz K" first="Karl-Josef" last="Dietz">Karl-Josef Dietz</name><affiliation wicri:level="1"><nlm:affiliation>Department of Biochemistry and Physiology of Plants, Bielefeld University, University Street 25, Bielefeld 33501, Germany.</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>Department of Biochemistry and Physiology of Plants, Bielefeld University, University Street 25, Bielefeld 33501</wicri:regionArea><wicri:noRegion>33501</wicri:noRegion><wicri:noRegion>33501</wicri:noRegion><wicri:noRegion>Bielefeld 33501</wicri:noRegion></affiliation></author></analytic><series><title level="j">Functional plant biology : FPB</title><idno type="eISSN">1445-4416</idno><imprint><date when="2013" type="published">2013</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Norway maple (Acer platanoides L., orthodox) and sycamore (Acer pseudoplatanus L., recalcitrant) belong to the same genus and grow under similar climatic conditions, but their seeds differ in their tolerance to desiccation. The initial water content (WC) of the seeds used in this study was 50%, and they were dried to 40, 20 and 7%. The mitochondrial peroxiredoxin IIF (PRXIIF) was identified in seeds of both species by immunoblotting. Semiquantitative RT-PCR analyses indicated that the transcript level of PRXIIF in both types of seeds increased during different stages of desiccation and was higher in seeds of Norway maple than in sycamore. General proteome analyses showed important differences between orthodox and recalcitrant seeds. In sycamore seeds that had been desiccated to a 7% WC, the number of protein spots and the levels of those spots were lower than in desiccation-tolerant Norway maple seeds. Post-translational modifications of PRXIIF in seeds at a 50% WC were detected via 2D electrophoresis and subsequent western blot analysis. The detected shift in the pI values (± 0.3) in A. pseudoplatanus was possibly caused by phosphorylation because several potential phosphorylation sites were predicted in silico for that protein. The gene and amino acid sequences were obtained and aligned with known sequences of other plant PRXIIF genes and proteins. High values of sequence identity were noted between the PRXIIF protein sequences of Acer species, Populus trichocarpa Torr. & A. Gray and Arabidopsis thaliana (L.) Heynh. The involvement of PRXIIF in defining the physiological differences between desiccation-tolerant and desiccation-sensitive Acer seeds is discussed in the context of its role in mitochondrial redox homeostasis.</div></front></TEI><pubmed><MedlineCitation Status="PubMed-not-MEDLINE" Owner="NLM"><PMID Version="1">32481169</PMID><DateRevised><Year>2020</Year><Month>06</Month><Day>02</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Electronic">1445-4416</ISSN><JournalIssue CitedMedium="Internet"><Volume>40</Volume><Issue>10</Issue><PubDate><Year>2013</Year><Month>Oct</Month></PubDate></JournalIssue><Title>Functional plant biology : FPB</Title><ISOAbbreviation>Funct Plant Biol</ISOAbbreviation></Journal><ArticleTitle>The involvement of the mitochondrial peroxiredoxin PRXIIF in defining physiological differences between orthodox and recalcitrant seeds of two Acer species.</ArticleTitle><Pagination><MedlinePgn>1005-1017</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1071/FP13002</ELocationID><Abstract><AbstractText>Norway maple (Acer platanoides L., orthodox) and sycamore (Acer pseudoplatanus L., recalcitrant) belong to the same genus and grow under similar climatic conditions, but their seeds differ in their tolerance to desiccation. The initial water content (WC) of the seeds used in this study was 50%, and they were dried to 40, 20 and 7%. The mitochondrial peroxiredoxin IIF (PRXIIF) was identified in seeds of both species by immunoblotting. Semiquantitative RT-PCR analyses indicated that the transcript level of PRXIIF in both types of seeds increased during different stages of desiccation and was higher in seeds of Norway maple than in sycamore. General proteome analyses showed important differences between orthodox and recalcitrant seeds. In sycamore seeds that had been desiccated to a 7% WC, the number of protein spots and the levels of those spots were lower than in desiccation-tolerant Norway maple seeds. Post-translational modifications of PRXIIF in seeds at a 50% WC were detected via 2D electrophoresis and subsequent western blot analysis. The detected shift in the pI values (± 0.3) in A. pseudoplatanus was possibly caused by phosphorylation because several potential phosphorylation sites were predicted in silico for that protein. The gene and amino acid sequences were obtained and aligned with known sequences of other plant PRXIIF genes and proteins. High values of sequence identity were noted between the PRXIIF protein sequences of Acer species, Populus trichocarpa Torr. & A. Gray and Arabidopsis thaliana (L.) Heynh. The involvement of PRXIIF in defining the physiological differences between desiccation-tolerant and desiccation-sensitive Acer seeds is discussed in the context of its role in mitochondrial redox homeostasis.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Ratajczak</LastName><ForeName>Ewelina</ForeName><Initials>E</Initials><AffiliationInfo><Affiliation>Institute of Dendrology, Polish Academy of Sciences, Seed Biochemistry Laboratory, Parkowa 5, 62-035 Kórnik, Poland.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Str Her</LastName><ForeName>Elke</ForeName><Initials>E</Initials><AffiliationInfo><Affiliation>Department of Biochemistry and Physiology of Plants, Bielefeld University, University Street 25, Bielefeld 33501, Germany.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Oelze</LastName><ForeName>Marie-Luise</ForeName><Initials>ML</Initials><AffiliationInfo><Affiliation>Department of Biochemistry and Physiology of Plants, Bielefeld University, University Street 25, Bielefeld 33501, Germany.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Kalemba</LastName><ForeName>Ewa M</ForeName><Initials>EM</Initials><AffiliationInfo><Affiliation>Institute of Dendrology, Polish Academy of Sciences, Seed Biochemistry Laboratory, Parkowa 5, 62-035 Kórnik, Poland.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Pukacka</LastName><ForeName>Stanis Awa</ForeName><Initials>SA</Initials><AffiliationInfo><Affiliation>Institute of Dendrology, Polish Academy of Sciences, Seed Biochemistry Laboratory, Parkowa 5, 62-035 Kórnik, Poland.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Dietz</LastName><ForeName>Karl-Josef</ForeName><Initials>KJ</Initials><AffiliationInfo><Affiliation>Department of Biochemistry and Physiology of Plants, Bielefeld University, University Street 25, Bielefeld 33501, Germany.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>Australia</Country><MedlineTA>Funct Plant Biol</MedlineTA><NlmUniqueID>101154361</NlmUniqueID><ISSNLinking>1445-4416</ISSNLinking></MedlineJournalInfo><CitationSubset>IM</CitationSubset></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2013</Year><Month>01</Month><Day>02</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2013</Year><Month>03</Month><Day>27</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2020</Year><Month>6</Month><Day>3</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2013</Year><Month>10</Month><Day>1</Day><Hour>0</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2013</Year><Month>10</Month><Day>1</Day><Hour>0</Hour><Minute>1</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">32481169</ArticleId><ArticleId IdType="pii">FP13002</ArticleId><ArticleId IdType="doi">10.1071/FP13002</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Allemagne</li><li>Pologne</li></country></list><tree><country name="Pologne"><noRegion><name sortKey="Ratajczak, Ewelina" sort="Ratajczak, Ewelina" uniqKey="Ratajczak E" first="Ewelina" last="Ratajczak">Ewelina Ratajczak</name></noRegion><name sortKey="Kalemba, Ewa M" sort="Kalemba, Ewa M" uniqKey="Kalemba E" first="Ewa M" last="Kalemba">Ewa M. Kalemba</name><name sortKey="Pukacka, Stanis Awa" sort="Pukacka, Stanis Awa" uniqKey="Pukacka S" first="Stanis Awa" last="Pukacka">Stanis Awa Pukacka</name></country><country name="Allemagne"><noRegion><name sortKey="Str Her, Elke" sort="Str Her, Elke" uniqKey="Str Her E" first="Elke" last="Str Her">Elke Str Her</name></noRegion><name sortKey="Dietz, Karl Josef" sort="Dietz, Karl Josef" uniqKey="Dietz K" first="Karl-Josef" last="Dietz">Karl-Josef Dietz</name><name sortKey="Oelze, Marie Luise" sort="Oelze, Marie Luise" uniqKey="Oelze M" first="Marie-Luise" last="Oelze">Marie-Luise Oelze</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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